@article{10902/27789,
year = {2023},
url = {https://hdl.handle.net/10902/27789},
abstract = {Epithelial transdifferentiation is frequent in tissue hyperplasia and contributes to disease in various degrees. Squamous metaplasia (SQM) precedes epidermoid lung cancer, an aggressive and frequent malignancy, but it is rare in the epithelium of the mammary gland. The mechanisms leading to SQM in the lung have been very poorly investigated. We have studied this issue on human freshly isolated cells and organoids. Here we show that human lung or mammary cells strikingly undergo SQM with polyploidisation when they are exposed to genotoxic or mitotic drugs, such as Doxorubicin or the cigarette carcinogen DMBA, Nocodazole, Taxol or inhibitors of Aurora-B kinase or Polo-like kinase. To note, the epidermoid response was attenuated when DNA repair was enhanced by Enoxacin or when mitotic checkpoints where abrogated by inhibition of Chk1 and Chk2. The results show that DNA damage has the potential to drive SQM via mitotic checkpoints, thus providing novel molecular candidate targets to tackle lung SCC. Our findings might also explain why SCC is frequent in the lung, but not in the mammary gland and why chemotherapy often causes complicating skin toxicity.},
organization = {ACKNOWLEDGEMENTS: We thank Darío Alves and Ángel Saiz for technical assistance and Johan Van Es for support. This work was funded by Instituto de Salud Carlos III (ISCIII)-FEDER, grants PI17/03107 and PI20/00880 (AG; Spain), the Netherlands Organ-on-Chip Initiative, NWO Gravitation project 024.003.001 and by the Ministry of Education, Culture and Science of the government of the Netherlands (HC; The Netherlands). LSJ and NSG were supported by University of Cantabria/IDIVAL fellowships PREVAL 19/06 and 16/04, respectively. CPM was supported by the aforementioned project 024.003.001 (The Netherlands).},
publisher = {Springer Nature},
publisher = {Cell Death Discovery, 2023, 9(1), 21},
title = {DNA damage triggers squamous metaplasia in human lung and mammary cells via mitotic checkpoints},
author = {San Juan, Lucía and Freije, Ana and Sanz-Gómez, Natalia and Jiménez-Matías, Beatriz and Pleguezuelos-Manzano, Cayetano and Sanz Giménez-Rico, Juan Ramón and Diego García, Ernesto Matías de and Naranjo Gozalo, Sara and Clevers, Hans and Gandarillas, Alberto},
}