@article{10902/27232,
year = {2022},
url = {https://hdl.handle.net/10902/27232},
abstract = {Osteoporosis (OP) is characterized by a loss in bone mass and mineral density. The stimulation of the canonical Wnt/?-catenin pathway has been reported to promote bone formation, this pathway is controlled by several regulators as secreted frizzled-related protein-1 (Sfrp-1), antagonist of the pathway. Thus, Sfrp-1 silencing therapies could be suitable for enhancing bone growth. However, the systemic stimulation of Wnt/?-catenin has been correlated with side effects. This work hypothesizes the administration of lipid-polymer NPs (LPNPs) functionalized with a MSC specific aptamer (Apt) and carrying a SFRP1 silencing GapmeR, could favor bone formation in OP with minimal undesired effects. Suitable SFRP1 GapmeR-loaded Apt-LPNPs (Apt-LPNPs-SFRP1) were administered in osteoporotic mice and their biodistribution, toxicity and bone induction capacity were evaluated. The aptamer functionalization of the NPs modified their biodistribution profile showing a four-fold increase in the bone accumulation and a ten-fold decrease in the hepatic accumulation compared to naked LPNPs. Moreover, the histological evaluation revealed evident changes in bone structure observing a more compact trabecular bone and a cortical bone thickness increase in the Apt-LPNPs-SFRP1 treated mice with no toxic effects. Therefore, these LPNPs showed suitable properties and biodistribution profiles leading to an enhancement on the bone density of osteoporotic mice.},
organization = {Funding: This work is part of the project RTI2018-097324-B-100 funded by MCIN/AEI/10.13039/501100011033 and by ERDF “A way of making Europe”. Patricia García-García thanks the University of La Laguna for her research grant (M-ULL).},
publisher = {BioMed Central - Springer Nature},
publisher = {J Nanobiotechnology
. 2022 Oct 29;20(1):462},
title = {Nanoparticle-mediated selective Sfrp-1 silencing enhances bone density in osteoporotic mice},
author = {García-García, Patricia and Reyes, Ricardo and García Sánchez, Daniel and Pérez Campo, Flor María and Rodríguez Rey, José Carlos and Évora, Carmen and Díaz-Rodríguez, Patricia and Delgado, Araceli},
}