@article{10902/24848,
year = {2010},
month = {4},
url = {http://hdl.handle.net/10902/24848},
abstract = {Background The transcription factor Snail1 induces epithelial-to-mesenchymal transition (EMT), a process responsible for the acquisition of invasiveness during tumorigenesis. Several transcriptomic studies have reported Snail1-regulated genes in different cell types, many of them involved in cell adhesion. However, only a few studies have used proteomics as a tool for the characterization of proteins mediating EMT. Methodology/Principal Findings We identified by proteomic analysis using 2D-DIGE electrophoresis combined with MALDI-TOF-TOF and ESI-linear ion trap mass spectrometry a number of proteins with variable functions whose expression is modulated by Snail1 in SW480-ADH human colon cancer cells. Validation was performed by Western blot and immunofluorescence analyses. Snail1 repressed several members of the 14-3-3 family of phosphoserine/phosphothreonine binding proteins and also the expression of the Proliferation-associated protein 2G4 (PA2G4) that was mainly localized at the nuclear Cajal bodies. In contrast, the expression of two proteins involved in RNA processing, the Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) and the Splicing factor proline/glutaminerich (SFPQ), was higher in Snail1-expressing cells than in controls. The regulation of 14-3-3?, 14-3-3?, 14-3-3? and PA2G4 by Snail1 was reproduced in HT29 colon cancer cells. In addition, we found an inverse correlation between 14-3-3? and Snail1 expression in human colorectal tumors. Conclusions/Significance We have identified a set of novel Snail1 target proteins in colon cancer that expand the cellular processes affected by Snail1 and thus its relevance for cell function and phenotype.},
organization = {Funding: This work was supported by the Ministerio de Ciencia e Innovación of Spain (SAF2007-60341, BIO2006-07689, ISCIII-RETIC RD06/0020/0009 and RD06/0020/0040), Comunidad de Madrid (S-GEN-0266/2006) and the European Union (MRTN-CT-2005-019496, NucSys). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.},
organization = {Acknowledgments: We are grateful to T. Martínez and to the staff of the Immunohistochemistry Unit of the Centro Nacional de Investigaciones Oncológicas (Madrid, Spain) for technical assistance.},
publisher = {Public Library of Science},
publisher = {PLoS One
. 2010 Apr 20;5(4):e10221},
title = {Novel Snail1 Target Proteins in Human Colon Cancer Identified by Proteomic Analysis},
author = {Larriba, María Jesús and Casado-Vela, Juan and Prendás-Franco, Natalia and Peña, Raúl and García de Herreros, Antonio and Berciano Blanco, María Teresa and Lafarga Coscojuela, Miguel Ángel and Casal Boo, Ignacio Jefferson and Muñoz Solano, Alberto},
}