@article{10902/24844,
year = {2021},
url = {http://hdl.handle.net/10902/24844},
abstract = {Vaccine efficacy is based on clinical data. Currently, the assessment of immune response after SARS-CoV-2 vaccination is scarce. A total of 52 healthcare workers were immunized with the same lot of BNT162b2 vaccine. The immunological response against the vaccine was tested using a T-specific assay based on the expression of CD25 and CD134 after stimulation with anti-N, -S, and -M specific peptides of SARS-CoV-2. Moreover, IgG anti-S2 and -RBD antibodies were detected using ELISA. Furthermore, the cell subsets involved in the response to the vaccine were measured in peripheral blood by flow cytometry. Humoral-specific responses against the vaccine were detected in 94% and 100% after the first and second doses, respectively. Therefore, anti-S T-specific responses were observed in 57% and 90% of the subjects after the first and second doses of the vaccine, respectively. Thirty days after the second dose, significant increases in T helper 1 memory cells (p < 0.001), peripheral memory T follicular helper (pTFH) cells (p < 0.032), and switched memory (p = 0.005) were observed. This study describes the specific humoral and cellular immune responses after vaccination with the new mRNA-based BNT162b2 vaccine. A mobilization of TFH into the circulation occurs, reflecting a specific activation of the immune system.},
organization = {Funding: This work was partially supported by the Cantabrian Government, grant number 2020UIC22-PUB-001, and from Instituto de Salud Carlos III, grant number COV20/00170.},
publisher = {MDPI AG},
publisher = {Biomedicines
. 2021 Jul 22;9(8):868},
title = {Immune Assessment of BNT162b2 m-RNA-Spike Based Vaccine Response in Adults},
author = {San Segundo, David and Comins-Boo, Alejandra and Irure Ventura, Juan and Renuncio-García, Mónica and Roa-Bautista, Adriel and González-López, Elena and Merino-Fernández, David and Lamadrid-Perojo, Patricia and Alonso-Peña, Marta and Ocejo-Vinyals, Javier Gonzalo and Gutiérrez-Larrañaga, María and Guiral-Foz, Sandra and López Hoyos, Marcos},
}