@article{10902/23772,
year = {2021},
url = {http://hdl.handle.net/10902/23772},
abstract = {Bacterial conjugation is the main mechanism for horizontal gene transfer, conferring plasticity to the genome repertoire. This process is also the major instrument for the dissemination of antibiotic resistance genes. Hence, gathering primary information of the mechanism underlying this genetic transaction is of a capital interest. By using fluorescent protein fusions to the ATPases that power conjugation, we have been able to track the localization of these proteins in the presence and absence of recipient cells. Moreover, we have found that more than one copy of the conjugative plasmid is transferred during mating. Altogether, these findings provide new insights into the mechanism of such an important gene transfer device.},
organization = {This work was supported by the Spanish Ministerio de Ciencia e Innovación (MINECO) Grants BFU2016-78521-R and PID2019-104251GB-I00.},
publisher = {Frontiers Research Foundation},
publisher = {Front Microbiol
. 2021 Oct 4;12:750200},
title = {Monitoring bacterial conjugation by optical microscopy},
author = {Carranza, Gerardo and Menguiano Vázquez, Tamara and Valenzuela Gómez, Fernando and García Cazorla, Yolanda and Cabezón Navarro, María Elena and Arechaga Iturregui, Ignacio María},
}