@article{10902/20273,
year = {2020},
url = {http://hdl.handle.net/10902/20273},
abstract = {Rapid cryopreservation of biological specimens is the gold standard for visualizing cellular structures in their true structural context. However, current commercial cryo-fluorescence microscopes are limited to low resolutions. To fill this gap, we have developed cryoSIM, a microscope for 3D super-resolution fluorescence cryo-imaging for correlation with cryo-electron microscopy or cryo-soft X-ray tomography. We provide the full instructions for replicating the instrument mostly from off-the-shelf components and accessible, user-friendly, open-source Python control software. Therefore, cryoSIM democratizes the ability to detect molecules using super-resolution fluorescence imaging of cryopreserved specimens for correlation with their cellular ultrastructure.},
organization = {Funding: Wellcome Trust (091911/Z/11/Z, 096144/Z/11/Z, 105605/Z/14/Z, 107457/Z/15/Z, 203141/Z/16/Z, 209412/Z/17/Z); H2020Marie Skłodowska-Curie Actions (700184).},
publisher = {Optical Society of America},
publisher = {Optica. 2020 (7): 802-812},
title = {CryoSIM: super-resolution 3D structured illumination cryogenic fluorescence microscopy for correlated ultrastructural imaging},
author = {Phillips, Michael A. and Harkiolaki, Maria and Susano Pinto, David Miguel and Parton, Richard M. and Palanca Cuñado, Ana Rosa and García Moreno, Manuel and Kounatidis, Ilias and Sedat, John W. and Stuart, David I. and Castello, Alfredo and Booth, Martin J. and Davis, Ilan and Dobbie, Ian M.},
}