@article{10902/17587,
year = {2019},
month = {3},
url = {http://hdl.handle.net/10902/17587},
abstract = {TraB is an FtsK-like DNA translocase responsible for conjugative plasmid transfer in mycelial Streptomyces Unlike other conjugative systems, which depend on a type IV secretion system, Streptomyces requires only TraB protein to transfer the plasmid as dsDNA. The ?-domain of this protein specifically binds to repeated 8-bp motifs on the plasmid sequence, following a mechanism that is reminiscent of the FtsK/SpoIIIE chromosome segregation system. In this work, we purified and characterized the enzymatic activity of TraB, revealing that it is a DNA-dependent ATPase that is highly stimulated by dsDNA substrates. Interestingly, we found that unlike the SpoIIIE protein, the ?-domain of TraB does not confer sequence-specific ATPase stimulation. We also found that TraB binds G-quadruplex DNA structures with higher affinity than TraB-recognition sequences (TRSs). An EM-based structural analysis revealed that TraB tends to assemble as large complexes comprising four TraB hexamers, which might be a prerequisite for DNA translocation across cell membranes. In summary, our findings shed light on the molecular mechanism used by the DNA-translocating motor TraB, which may be shared by other membrane-associated machineries involved in DNA binding and translocation.},
organization = {Funding: This work was supported by the Spanish Ministerio de Economia y Competitividad (MINECO) grants BFU2016-78521-R (to E.C. and I.A.) and Deutsche Forschungsgemeinschaft SFB766 (to G.M).},
publisher = {American Society for Biochemistry and Molecular Biology Inc.},
publisher = {J Biol Chem. 2019 Mar 29;294(13):5050-5059},
title = {The FtsK-like motor TraB is a DNA-dependent ATPase that forms higher-order assemblies},
author = {Amado, Eric and Muth, Gunther and Arechaga Iturregui, Ignacio María and Cabezón Navarro, María Elena},
}