@article{10902/1734,
year = {2011},
month = {11},
url = {http://hdl.handle.net/10902/1734},
abstract = {Biomarkers are indicators of biological processes and hold promise for the diagnosis and treatment of disease. Gliomas represent a heterogeneous group of brain tumors with marked intra- and inter-tumor variability. The extent of surgical resection is a significant factor influencing post-surgical recurrence and prognosis. Here, we used fluorescence and reflectance spectral signatures for in vivo quantification of multiple biomarkers during glioma surgery, with fluorescence contrast provided by exogenously-induced protoporphyrin IX (PpIX) following administration of 5-aminolevulinic acid. We performed light-transport modeling to quantify multiple biomarkers indicative of tumor biological processes, including the local concentration of PpIX and associated photoproducts, total hemoglobin concentration, oxygen saturation, and optical scattering parameters.We developed a diagnostic algorithm for intra-operative tissue delineation that accounts for the combined tumor-specific predictive capabilities of these quantitative biomarkers. Tumor tissue delineation achieved accuracies of up to 94% (specificity=94%, sensitivity=94%) across a range of glioma histologies beyond current state-of-the-art optical approaches, including state-of-the-art fluorescence image guidance. This multiple biomarker strategy opens the door to optical methods for surgical guidance that use quantification of well-established neoplastic processes. Future work would seek to validate the predictive power of this proof-of-concept study in a separate larger cohort of patients.},
publisher = {SPIE Society of Photo-Optical Instrumentation Engineers},
publisher = {Journal of Biomedical Optics, 2011, 16(11), 116007},
title = {Combined fluorescence and reflectance spectroscopy for in vivo quantification of cancer biomarkers in low - and high- grade glioma surgery},
author = {Valdés, Pablo A. and Kim, Anthony and Leblond, Frederic and Conde Portilla, Olga María and Harris, Brent T. and Paulsen, Keith D. and Wilson, Brian C. and Roberts, David W.},
}