@article{10902/15470,
year = {2018},
month = {1},
url = {http://hdl.handle.net/10902/15470},
abstract = {Melatonin exerts oncostatic activity in breast cancer through antiangiogenic actions. There, the aim of the present study was to ascertain whether melatonin modulates, in a coordinated action, angiopoietin-1 (ANG-1), ANG-2, their cognate Tie2 receptor and VEGF in co-cultures of human endothelial cells (HUVECs) and breast cancer (MCF-7) cells. To accomplish this we used co-cultures of human breast cancer cells (MCF-7) or non-malignant human mammary epithelial cells (MCF?10A) with endothelial cells (HUVECs). The presence of breast cancer cells increased HUVEC proliferation and 1 mM melatonin prevented this effect. ANG-1, ANG-2 and VEGF levels in co-culture media and mRNA expression were upregulated and Tie2 mRNA expression was downregulated in the HUVECs and MCF-7. Melatonin (1 mM) downregulated ANG-1, ANG-2 and VEGF levels in the co-culture media and mRNA expression in both types of cells and upregulated Tie2 mRNA expression in HUVECs. ANG-1, ANG-2, Tie2 and VEGF mRNA expression were not modified during HUVEC/MCF-10A co-culture. Estradiol (10 nM) increased ANG-1, ANG-2 and VEGF mRNA expression in HUVECs and melatonin (1 mM) counteracted this effect. We conclude that melatonin simultaneously coordinates downregulation of angiopoietins with a reduction in VEGF, which could be an effective therapeutic strategy for blocking tumor angiogenesis.},
organization = {The present study was supported by grants from the Spanish Economy and Competitiveness Ministry (SAF2013-42012-P, SAF2016-77103-P), and from the Instituto de Investigación
Sanitaria Valdecilla (IDIVAL) (APG/12).},
publisher = {Spandidos Publications},
publisher = {Oncol Rep. 2018 Jan;39(1):433-441},
title = {Complementary actions of melatonin on angiogenic factors,the angiopoietin/Tie2 axis and VEGF, in co-cultures of human endothelial and breast cancer cells},
author = {González González, Alicia and González Cabeza, Alicia Verónica and Alonso González, Carolina and Menéndez Menéndez, Javier and Martínez Campa, Carlos Manuel and Cos Corral, Samuel},
}