@article{10902/13070,
year = {2017},
url = {http://hdl.handle.net/10902/13070},
abstract = {The definition of anti-HLA antibody profile in highly sensitized patients on a waiting list is crucial when virtual crossmatch is used in organ allocation systems, but also when used to identify the true deleterious anti-HLA antibodies. Here we propose different levels of risk based on the results of anti-HLA antibody testing in neat serum (N) and after sera dilution (DIL) and C1q test in 18 highly sensitized patients. This group was heterogeneous in terms of anti-HLA antibody titers and their ability to fix complement. After dilution, 15 out of 18 patients (83.3%) showed a reduction of positive bead counts whereas 4 patients showed a prozone effect and complement fixation was demonstrated. The high dilution of sera and ascertaining the complement fixation allow the accurate definition of risk anti-HLA antibody profiles in highly sensitized patients, as demonstrated in 5 of the sensitized patients who were transplanted.},
publisher = {Public Library of Science},
publisher = {PLoS One. 2017; 12(2): e0171463},
title = {Improvement in the definition of anti-HLA antibody profile in highly sensitized patients},
author = {Irure Ventura, Juan and Asensio, Esther and Rodrigo Calabia, Emilio and Romón Alonso, José Iñigo and Gómez, Javier and Arias Rodríguez, Manuel and López Hoyos, Marcos and San Segundo Arribas, David},
}