@article{10902/11932,
year = {2017},
url = {http://hdl.handle.net/10902/11932},
abstract = {We report the development of new chemical probes for cereulide,
a toxic metabolite produced by specific strains of Bacillus
cereus, through displacement of potassium cations from a preformed
specific complex and a subsequent change in the fluorescence
emission. For this purpose, we designed fluorescent
probes for potassium cations that were suitable for displacement
assays with cereulide from organic extracts. The fluorescence
detection of natural cereulide in rice samples was achieved
by using synthetic cereulide as a reference and a potassium
fluorescent reporter, and this was found to be useful as
a portable and fast method for the in situ detection of cereulide
in food extracts. To study the fate of cereulide in live cells,
we designed a procedure that was suitable for live-cell microscopy
imaging of HeLa cells by comparing the cellular location
of the potassium fluorogenic probe, which stained intracellular
endolysosomes, in the absence and presence of cereulide; we
concluded that in the presence of cereulide, the fluorescence
of the probe was decreased because of complexation of the
potassium ions by cereulide.},
organization = {We gratefully acknowledge financial support from the Ministerio de Economía y Competitividad, Spain (Projects CTQ2015-71353-R and AES-PI16/000496), Junta de Castilla y León, Consejería de Educación y Cultura y Fondo Social Europeo (Project BU232U13), and the European Commission, Seventh Framework Programme (Project SNIFFER FP7-SEC-2012–312411). J. G.-C. thanks Ministerio de Economía y Competitividad for his predoctoral FPU fellowship.},
publisher = {Wiley},
publisher = {ChemistryOpen, 2017, 6(4), 562-570},
title = {Potassium-ion-selective fluorescent sensors to detect cereulide, the emetic toxin of B. cereus, in food samples and HeLa cells},
author = {García Calvo, José and Ibeas, Saturnino and Antón García, Eva Clara and Torroba, Tomás and González Aguilar, Gerardo and Antunes, Wilson and González Lavado, Eloisa and López Fanarraga, Mónica},
}